Introduction to Plant Biotechnology
by H. S. Chawla
Plant biotechnology has created unprecedented opportunities for the manipulation of biological systems of plants. To understand biotechnology, it is essential to know the basic aspects of genes and their organization in the genome of plant cells. This text on the subject is aimed at students.
by H. S. Chawla
Basics; Laboratory organization; Sterilization techniques; Nutrition medium; Choice of the explant; Plant tissue culture; Seed culture; Micropropagation- meristem culture; Micropropagation- axillary bud proliferation; Micropropagation- adventitious regeneration; Micropropagation- organogenesis; Micropropagation- embryogenesis; Cell suspension; Secondary metabolite production in a cell suspension culture; Anther culture; Protoplast isolation and fusion; Biotechnology; SDS-PAGE electrophoresis of proteins; Isolation of DNA from plant tissues; Isolation an purification of plasmid DNA; Restriction enzyme digestion of DNA; Agarose gel electrophoresis; Preparation of competent cells, transformation of E. coil with plasmid DNA and ligation of insert DNA to a vector; Agrobacterium-mediated gene transfer; Biolistic method of transformation in plants; In vitro amplification of DNA by PCR: detection of transgenes; RAPD analysis; Microsatellite marker analysis; Southerm biotting; Southerm hybridization.
Introduction to Plant Tissue Culture
by M. K. Razdan
Introduction and techniques; Introductory history; Laboratory organisation; Media; Aseptic manipulation; Basic aspects; Cell culture; Cellular totipotency; Somatic embryogenesis; Applications to plant breeding; Haploid prodution; Triploid production; In vitro pollination and fertilization; Zygotic embryo culture; Somatic hybridisation and cybridisation; Genetic transformation; Somaclonal and gametoclonal variant selection; Application to horticulture and forestry; Production of disease-free plants; clonal propagation; General applications; Industrial applications: secondary metabolite production; Germplasm conservation.
by Adrian Slater, Nigel W. Scott, Mark R. Fowler
The purpose of this book is to provide an overview of the production of GM crops, highlighting the key scientific and technical advances that underpin their development. The text begins with a summary of current knowledge about plant genome organisation and gene expression, followed by anintroduction to the techniques of plant tissue culture and genetic transformation and their application to crop plants. A consideration of the design of constructs for plant genetic manipulation precedes a series of chapters covering specific targets for GM crops. These include the geneticmanipulation of herbicide resistance, pest resistance and disease resistance. Strategies for engineering stress tolerance and the improvement of crop yield and quality are discussed, and the prospects for “molecular farming” are considered. Key themes and strategies are developed using appropriatecase studies, which place the science in its broader agricultural/commercial context. The text concentrates on the core molecular biological issues, while the accompanying Online Resource Centre encourages an exploration of the wider implications and concerns about GM crops. Online Resource Centre All the figures from the book are available to download free from the Online Resource Centre. It also features weblinks to relevant sites with additional information or discussion including those hosted by the protagonists of the GM debate.